PMID:1646811

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Citation

Allen, GC Jr and Kornberg, A (1991) The priB gene encoding the primosomal replication n protein of Escherichia coli. J. Biol. Chem. 266:11610-3

Abstract

The gene encoding protein n of the Escherichia coli primosome has been discovered in the rpsF-rpsR-rplI ribosomal protein operon and designated priB. The low copy number of PriB protein and the distinctive codon usage of its gene argue against its being a ribosomal protein. A strain which overproduces PriB was constructed and has been used to purify the protein to homogeneity. The overproduced protein behaves like that purified from wild-type cells.

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Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Chromatography, Gel; Codon; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Molecular Sequence Data; Operon; Protein Biosynthesis; Ribosomal Proteins/genetics

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