PMID:16452619

Citation	Pimentel, C, Choi, SJ, Chagot, B, Guette, C, Camadro, JM and Darbon, H (2006) Solution structure of PcFK1, a spider peptide active against Plasmodium falciparum. Protein Sci. 15:628-34
Abstract	Psalmopeotoxin I (PcFK1) is a 33-amino-acid residue peptide isolated from the venom of the tarantula Psalmopoeus cambridgei. It has been recently shown to possess strong antiplasmodial activity against the intra-erythrocyte stage of Plasmodium falciparum in vitro. Although the molecular target for PcFK1 is not yet determined, this peptide does not lyse erythrocytes, is not cytotoxic to nucleated mammalian cells, and does not inhibit neuromuscular function. We investigated the structural properties of PcFK1 to help understand the unique mechanism of action of this peptide and to enhance its utility as a lead compound for rational development of new antimalarial drugs. In this paper, we have determined the three-dimensional solution structure by (1)H two-dimensional NMR means of recombinant PcFK1, which is shown to belong to the ICK structural superfamily with structural determinants common to several neurotoxins acting as ion channels effectors.
Links	PubMed PMC2249782 Online version:10.1110/ps.051860606
Keywords	Animals; Antimalarials/chemistry; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Peptides/chemistry; Plasmodium falciparum/drug effects; Solutions; Spider Venoms/chemistry
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.