PMID:16445752

Citation	Yu, BJ, Sung, BH, Lee, JY, Son, SH, Kim, MS and Kim, SC (2006) sucAB and sucCD are mutually essential genes in Escherichia coli. FEMS Microbiol. Lett. 254:245-50
Abstract	sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD, sucAB, and sucCD under the control of a P(BAD) in E. coli MG1655, E. coli MG1655 (DeltasucCD), and E. coli MG1655 (DeltasucAB), respectively. These strains grew well in Luria-Bertani medium containing 0.1% arabinose, but not in the absence of arabinose unless the medium was supplemented with succinyl-CoA. Our results indicate that either sucAB or sucCD is enough to produce succinyl-CoA that is essential for cell viability.
Links	PubMed Online version:10.1111/j.1574-6968.2005.00026.x
Keywords	Acyl Coenzyme A/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Essential; Ketoglutarate Dehydrogenase Complex/genetics; Ketoglutarate Dehydrogenase Complex/metabolism; Succinate-CoA Ligases/genetics; Succinate-CoA Ligases/metabolism
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