PMID:16428424
| Citation |
Froelich, JM, Tran, K and Wall, D (2006) A pmrA constitutive mutant sensitizes Escherichia coli to deoxycholic acid. J. Bacteriol. 188:1180-3 |
|---|---|
| Abstract |
An Escherichia coli mutant was isolated and shown to be polymyxin B resistant. Mapping and sequence analysis revealed a missense mutation at codon 53 within the pmrA (basR) gene that results in a G-to-V substitution. Fusions of promoters from the pmrC, yibD, and pmrH genes with the lacZ reporter showed that they were constitutively expressed in pmrA53 cells. In pmrA+ strains, these promoters were induced by iron and zinc, while a DeltapmrA mutation blocked induction. The PmrA regulon regulates genes whose products remodel the composition and charge of lipid A and hence the barrier properties of the outer membrane. Along these lines, the pmrA53 mutant was also found to be hypersensitive to the anionic bile detergent deoxycholic acid. |
| Links |
PubMed PMC1347340 Online version:10.1128/JB.188.3.1180-1183.2006 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Deoxycholic Acid/pharmacology; Drug Resistance, Bacterial; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/pathogenicity; Gene Expression Regulation, Bacterial; Lipid A/chemistry; Mutation; Polymyxin B/pharmacology |
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