PMID:16417496

Citation	Whitehead, RN and Cole, JA (2006) Different responses to nitrate and nitrite by the model organism Escherichia coli and the human pathogen Neisseria gonorrhoeae. Biochem. Soc. Trans. 34:111-4
Abstract	The ability of Escherichia coli to use both nitrate and nitrite as terminal electron acceptors during anaerobic growth is mediated by the dual-acting two-component regulatory systems NarX-NarL and NarQ-NarP. In contrast, Neisseria gonorrhoeae responds only to nitrite: it expresses only NarQ-NarP. We have shown that although N. gonorrhoeae NarQ can phosphorylate E. coli NarL and NarP, the N. gonorrhoeae NarP is unable to regulate gene expression in E. coli. Mutagenesis experiments have revealed residues in E. coli NarQ that are essential for nitrate and nitrite sensing. Chimaeric proteins revealed domains of NarQ that are important for ligand sensing.
Links	PubMed Online version:10.1042/BST0340111
Keywords	Anaerobiosis; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Humans; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutagenesis, Site-Directed; Neisseria gonorrhoeae/genetics; Neisseria gonorrhoeae/metabolism; Nitrates/metabolism; Nitrites/metabolism; Phosphoproteins/genetics; Phosphoproteins/metabolism; Protein Kinases/genetics; Protein Kinases/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Signal Transduction/physiology
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