PMID:16414046

Citation	Sauret-Güeto, S, Urós, EM, Ibáñez, E, Boronat, A and Rodríguez-Concepción, M (2006) A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase. FEBS Lett. 580:736-40
Abstract	The 2-C-methyl-D-erythritol 4-phosphate pathway has been proposed as a promising target to develop new antimicrobial agents. However, spontaneous mutations in Escherichia coli were observed to rescue the otherwise lethal loss of the first two enzymes of the pathway, 1-deoxy-D-xylulose 5-phosphate (DXP) synthase (DXS) and DXP reductoisomerase (DXR), with a relatively high frequency. A mutation in the gene encoding the E1 subunit of the pyruvate dehydrogenase complex was shown to be sufficient to rescue the lack of DXS but not DXR in vivo, suggesting that the mutant enzyme likely allows the synthesis of DXP or an alternative substrate for DXR.
Links	PubMed Online version:10.1016/j.febslet.2005.12.092
Keywords	Erythritol/analogs & derivatives; Erythritol/biosynthesis; Erythritol/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Mutation; Pentosephosphates/biosynthesis; Pentosephosphates/genetics; Protein Subunits/genetics; Protein Subunits/metabolism; Pyruvate Dehydrogenase Complex/genetics; Pyruvate Dehydrogenase Complex/metabolism; Sugar Phosphates/biosynthesis; Sugar Phosphates/genetics; Transferases/genetics
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