PMID:16412426

Citation	Butland, G, Zhang, JW, Yang, W, Sheung, A, Wong, P, Greenblatt, JF, Emili, A and Zamble, DB (2006) Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation. FEBS Lett. 580:677-81
Abstract	Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins. To analyze complex formation between these factors in Escherichia coli, they were genomically tagged and native multi-protein complexes were isolated. This method validated multiple interactions reported in separate studies from several organisms and defined a new complex containing the putative chaperone HybG and the large subunit of hydrogenase 1 or 2. The complex also includes HypE and HypD, which interact with each other before joining the larger complex.
Links	PubMed Online version:10.1016/j.febslet.2005.12.063
Keywords	Escherichia coli/enzymology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Targeting; Hydrogenase/biosynthesis; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Multiprotein Complexes
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.