PMID:16384101
| Citation |
Skoko, D, Yan, J, Johnson, RC and Marko, JF (2005) Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis. Phys. Rev. Lett. 95:208101 |
|---|---|
| Abstract |
We report single-DNA-stretching experiments showing that the protein Fis, an abundant bacterial chromosome protein of E. coli, mediates a dramatic DNA condensation to zero length. This condensation occurs abruptly when DNA tension is reduced below a protein-concentration-dependent threshold f* < 1 pN. Following condensation, reopening under larger forces proceeds via a series of discrete jumps, indicating that Fis is able to stabilize DNA crossings. Our experiments suggest that Fis may play a role in vivo stabilizing the "loop-domain" structure of the bacterial chromosome. |
| Links | |
| Keywords |
DNA, Bacterial/chemistry; Escherichia coli Proteins/chemistry; Factor For Inversion Stimulation Protein; Shear Strength; Transcription Factors/chemistry |
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