PMID:16377615
| Citation |
Drees, JC, Chitteni-Pattu, S, McCaslin, DR, Inman, RB and Cox, MM (2006) Inhibition of RecA protein function by the RdgC protein from Escherichia coli. J. Biol. Chem. 281:4708-17 |
|---|---|
| Abstract |
The Escherichia coli RdgC protein is a potential negative regulator of RecA function. RdgC inhibits RecA protein-promoted DNA strand exchange, ATPase activity, and RecA-dependent LexA cleavage. The primary mechanism of RdgC inhibition appears to involve a simple competition for DNA binding sites, especially on duplex DNA. The capacity of RecA to compete with RdgC is improved by the DinI protein. RdgC protein can inhibit DNA strand exchange catalyzed by RecA nucleoprotein filaments formed on single-stranded DNA by binding to the homologous duplex DNA and thereby blocking access to that DNA by the RecA nucleoprotein filaments. RdgC protein binds to single-stranded and double-stranded DNA, and the protein can be visualized on DNA using electron microscopy. RdgC protein exists in solution as a mixture of oligomeric states in equilibrium, most likely as monomers, dimers, and tetramers. This concentration-dependent change of state appears to affect its mode of binding to DNA and its capacity to inhibit RecA. The various species differ in their capacity to inhibit RecA function. |
| Links |
PubMed Online version:10.1074/jbc.M513592200 |
| Keywords |
Adenosine Triphosphatases/chemistry; Anisotropy; Bacterial Proteins/chemistry; Bacteriophages/metabolism; Binding Sites; Binding, Competitive; Cloning, Molecular; DNA/chemistry; DNA, Single-Stranded/chemistry; Dose-Response Relationship, Drug; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli Proteins/metabolism; Gene Deletion; Hydrolysis; Microscopy, Electron; Protein Binding; Rec A Recombinases/chemistry; Rec A Recombinases/metabolism; Recombination, Genetic; Serine Endopeptidases/chemistry; Spectrophotometry; Temperature; Time Factors |
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