PMID:16335997

Citation	Han, MJ, Lee, JW and Lee, SY Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins. J. Proteome Res. 4:2429-34
Abstract	Proteolytic degradation is one of the critical problems in two-dimensional electrophoresis (2-DE). Here we report that small heat shock proteins (sHsps), including IbpA(Ec) and IbpB(Ec) from Escherichia coli and Hsp26(Sc) from Saccharomyces cerevisiae, are able to protect proteins in vitro from proteolytic degradation. Addition of sHsps during 2-DE of human serum or whole cell extracts of E. coli, Mannheimia succinciproducens, Arabidopsis thaliana, and human kidney cells allowed detection of up to 50% more protein spots than those obtainable with currently available protease inhibitors. Therefore, the use of sHsps during 2-DE significantly improves proteome profiling by generally enabling the detection of many more protein spots that could not be seen previously.
Links	PubMed Online version:10.1021/pr050259m
Keywords	Animals; Arabidopsis/metabolism; Cell Line; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/metabolism; Heat-Shock Proteins/chemistry; Humans; Mannheimia/metabolism; Proteins/chemistry; Proteome; Proteomics/methods; Saccharomyces cerevisiae/metabolism
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.