PMID:1631098
| Citation |
Almassy, RJ, Janson, CA, Kan, CC and Hostomska, Z (1992) Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase. Proc. Natl. Acad. Sci. U.S.A. 89:6114-8 |
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| Abstract |
The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-A resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-A resolution. The structure is a modified doubly wound alpha/beta sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design. |
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| Keywords |
Acyltransferases/antagonists & inhibitors; Acyltransferases/metabolism; Acyltransferases/ultrastructure; Apoproteins/ultrastructure; Binding Sites; Computer Graphics; Crystallography; Escherichia coli/enzymology; Hydrogen Bonding; Hydroxymethyl and Formyl Transferases; Models, Molecular; Phosphoribosylglycinamide Formyltransferase; Protein Conformation; Recombinant Proteins; X-Ray Diffraction |
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