PMID:1630317

Citation	Nakashima, K, Sugiura, A, Momoi, H and Mizuno, T (1992) Phosphotransfer signal transduction between two regulatory factors involved in the osmoregulated kdp operon in Escherichia coli. Mol. Microbiol. 6:1777-84
Abstract	The proteins KdpD and KdpE are regulatory factors critically involved in the osmotic regulation of the kdpABC operon that is responsible for a high-affinity transport system in Escherichia coli. In this study, we obtained biochemical evidence supporting the view that the KdpD protein is a sensory protein kinase that exhibits autophosphorylation and KdpE-phosphotransfer characteristics. During the course of such studies we established a procedure for purifying the KdpE protein in large quantities. We also developed a procedure for preparing cytoplasmic membrane enriched with the KdpD protein that exhibits in vitro ability with regard to phosphorylation of KdpE protein.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Biological Transport; Cell Membrane/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Molecular Weight; Operon; Osmolar Concentration; Phosphorylation; Potassium/metabolism; Protein Kinases/genetics; Protein Kinases/isolation & purification; Protein Kinases/metabolism; Signal Transduction; Trans-Activators/genetics; Trans-Activators/isolation & purification; Trans-Activators/metabolism
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