PMID:16302275

Citation	Strocchi, M, Ferrer, M, Timmis, KN and Golyshin, PN (2006) Low temperature-induced systems failure in Escherichia coli: insights from rescue by cold-adapted chaperones. Proteomics 6:193-206
Abstract	The growth of Escherichia coli cells is impaired at temperatures below 21 degrees C and stops at 7.5 degrees C; however, growth of a transgenic strain producing the cold-adapted chaperones Cpn60 and Cpn10 from the psychrophilic bacterium Oleispira antarctica is good at low temperatures. The E. coli cpn(+) transgene offers a novel opportunity for examining the essential protein for cell viability at low temperatures. By screening a large-scale protein map (proteome) of cells of K-12 and its Cpn(+) transgene incubated at 4 degrees C, we identified 22 housekeeping proteins involved in systems failure of E. coli when confronted with low temperature. Through co-immunoprecipitation of Cpn60, Northern blot, and in vitro refolding, we systematically identified that protein-chaperone interactions are key determinants of their protein functions at low temperatures. Furthermore, chromosomal gene deletion experiments suggest that the mechanism of cold-induced systems failure in E. coli is cold-induced inactivation of the GroELS chaperonins and the resulting failure to refold cold-inactivated Dps, ClpB, DnaK and RpsB proteins. These findings: (1) indicate the potential importance of chaperones in cold sensitivity, cold adaptation and cold tolerance in cellular systems, and (2) suggest the identity of a few key cold-sensitive chaperone-interacting proteins that get inactivated and ultimately cause systems failure in E. coli cells at low temperatures.
Links	PubMed Online version:10.1002/pmic.200500031
Keywords	Adaptation, Physiological; Algorithms; Blotting, Northern; Blotting, Western; Chromosomes, Bacterial; Cold Temperature; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Molecular Chaperones/physiology; Proteome; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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