PMID:16288462
| Citation |
Lu, WC, Wang, CZ, Yu, EW and Ho, KM (2006) Dynamics of the trimeric AcrB transporter protein inferred from a B-factor analysis of the crystal structure. Proteins 62:152-8 |
|---|---|
| Abstract |
The Escherichia coli AcrB multidrug transporter recognizes a wide range of toxic chemicals and actively extrudes them from cells. The molecular basis of multidrug transport in AcrB remains unknown. Herein, we describe normal mode analyses to study important regions for drug recognition and extrusion in this transporter. Based on the X-ray structure of AcrB, an elastic network model has been able to correct errors arising from crystal imperfection in the experimental B-factors. The results allow us to understand the functional dynamics of this membrane protein. It is expected that this technique can be applied to other membrane proteins with known structures. |
| Links |
PubMed PMC2705243 Online version:10.1002/prot.20743 |
| Keywords |
Crystallography; Crystallography, X-Ray; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Macromolecular Substances/chemistry; Membrane Proteins/chemistry; Models, Molecular; Multidrug Resistance-Associated Proteins/chemistry; Multidrug Resistance-Associated Proteins/metabolism; Protein Conformation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.