PMID:16216575
| Citation |
Gan, J, Tropea, JE, Austin, BP, Court, DL, Waugh, DS and Ji, X (2005) Intermediate states of ribonuclease III in complex with double-stranded RNA. Structure 13:1435-42 |
|---|---|
| Abstract |
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates. |
| Links |
PubMed Online version:10.1016/j.str.2005.06.014 |
| Keywords |
Base Sequence; Cloning, Molecular; Crystallography, X-Ray; Dimerization; Escherichia coli/genetics; Fourier Analysis; Models, Chemical; Models, Molecular; Mutation; Nucleic Acid Conformation; Protein Binding; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Subunits; RNA, Double-Stranded/chemistry; RNA, Double-Stranded/metabolism; Ribonuclease III/chemistry; Ribonuclease III/genetics; Ribonuclease III/metabolism; Spectrometry, Mass, Electrospray Ionization; Spectrum Analysis, Raman; Templates, Genetic; X-Ray Diffraction |
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