PMID:16204212

Citation	Briegel, KJ, Baker, A and Jain, C (2006) Identification and analysis of Escherichia coli ribonuclease E dominant-negative mutants. Genetics 172:7-15
Abstract	The Escherichia coli (E. coli) ribonuclease E protein (RNase E) is implicated in the degradation and processing of a large fraction of RNAs in the cell. To understand RNase E function in greater detail, we developed an efficient selection method for identifying nonfunctional RNase E mutants. A subset of the mutants was found to display a dominant-negative phenotype, interfering with wild-type RNase E function. Unexpectedly, each of these mutants contained a large truncation within the carboxy terminus of RNase E. In contrast, no point mutants that conferred a dominant-negative phenotype were found. We show that a representative dominant-negative mutant can form mixed multimers with RNase E and propose a model to explain how these mutants can block wild-type RNase E function in vivo.
Links	PubMed PMC1456196 Online version:10.1534/genetics.105.048553
Keywords	Blotting, Western; Endoribonucleases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Dominant; Models, Molecular; Mutation/genetics; RNA Stability; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Selection, Genetic; Sequence Deletion
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