PMID:16199751
| Citation |
Singh, NS, Das, G, Seshadri, A, Sangeetha, R and Varshney, U (2005) Evidence for a role of initiation factor 3 in recycling of ribosomal complexes stalled on mRNAs in Escherichia coli. Nucleic Acids Res. 33:5591-601 |
|---|---|
| Abstract |
Specific interactions between ribosome recycling factor (RRF) and elongation factor-G (EFG) mediate disassembly of post-termination ribosomal complexes for new rounds of initiation. The interactions between RRF and EFG are also important in peptidyl-tRNA release from stalled pre-termination complexes. Unlike the post-termination complexes (harboring deacylated tRNA), the pre-termination complexes (harboring peptidyl-tRNA) are not recycled by RRF and EFG in vitro, suggesting participation of additional factor(s) in the process. Using a combination of biochemical and genetic approaches, we show that, (i) Inclusion of IF3 with RRF and EFG results in recycling of the pre-termination complexes; (ii) IF3 overexpression in Escherichia coli LJ14 rescues its temperature sensitive phenotype for RRF; (iii) Transduction of infC135 (which encodes a functionally compromised IF3) in E.coli LJ14 generates a 'synthetic severe' phenotype; (iv) The infC135 and frr1 (containing an insertion in the RRF gene promoter) alleles synergistically rescue a temperature sensitive mutation in peptidyl-tRNA hydrolase in E.coli; and (v) IF3 facilitates ribosome recycling by Thermus thermophilus RRF and E.coli EFG in vivo and in vitro. These lines of evidence clearly demonstrate the physiological importance of IF3 in the overall mechanism of ribosome recycling in E.coli. |
| Links |
PubMed PMC1240113 Online version:10.1093/nar/gki864 |
| Keywords |
Alleles; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Mutation; Peptide Chain Initiation, Translational; Peptide Chain Termination, Translational; Peptide Elongation Factor G/metabolism; Phenotype; Prokaryotic Initiation Factor-3/genetics; Prokaryotic Initiation Factor-3/physiology; RNA, Messenger/metabolism; Ribosomal Proteins/physiology; Ribosomes/metabolism; Temperature |
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