PMID:16172544
| Citation |
Kikuchi, T, Mizunoe, Y, Takade, A, Naito, S and Yoshida, S (2005) Curli fibers are required for development of biofilm architecture in Escherichia coli K-12 and enhance bacterial adherence to human uroepithelial cells. Microbiol. Immunol. 49:875-84 |
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| Abstract |
Sessile bacteria show phenotypical, biochemical, and morphological differences from their planktonic counterparts. Curli, extracellular structures important for biofilm formation, are only produced at temperatures below 30 C in Escherichia coli K-12 strains. In this report, we show that E. coli K-12 can produce curli at 37 C when grown as a biofilm community. The curli-expressing strain formed more biofilms on polyurethane sheets than the curli-deficient strain under growth temperatures of both 25 C and 37 C. Curli are required for the formation of a three-dimensional mature biofilm, with characteristic water channels and pillars of bacteria. Observations by electron microscopy revealed the presence at the surfaces of the curli-deficient mutant in biofilm of flagella and type I pili. A wild-type curli-expressing E. coli strain significantly adhered to several lines of human uroepithelial cells, more so than an isogenic curlideficient strain. The finding that curli are expressed at 37 C in biofilm and enhance bacterial adherence to mammalian host cells suggests an important role for curli in pathogenesis. |
| Links | |
| Keywords |
Bacterial Adhesion/genetics; Bacterial Adhesion/physiology; Biofilms/growth & development; Cell Line; Colony Count, Microbial; Epithelial Cells/microbiology; Escherichia coli/pathogenicity; Escherichia coli/ultrastructure; Fimbriae, Bacterial/physiology; Fimbriae, Bacterial/ultrastructure; Flagella/ultrastructure; Gene Deletion; Humans; Temperature; Virulence Factors/genetics; Virulence Factors/physiology |
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