PMID:16171983
| Citation |
El-Mansi, M (2005) Free CoA-mediated regulation of intermediary and central metabolism: an hypothesis which accounts for the excretion of alpha-ketoglutarate during aerobic growth of Escherichia coli on acetate. Res. Microbiol. 156:874-9 |
|---|---|
| Abstract |
During growth of Escherichia coli on acetate, phosphotransacetylase and alpha-ketoglutarate dehydrogenase are in direct competition for their common co-factor, HS-CoA. Such competition is resolved in favour of phosphotransacetylase, thus rendering alpha-ketoglutarate dehydrogenase rate-limiting (controlling) and, in turn, creating a bottleneck at the level of alpha-ketoglutarate in the Krebs cycle. Accumulation of alpha-ketoglutarate is then balanced by its excretion. Addition of pyruvate, glucose or any glycolytic intermediate to acetate-grown culture relieves such a bottleneck by reversing carbon flow through phosphotransacetylase to give acetyl phosphate and much-needed HS-CoA. The urgent need for HS-CoA by the primordial organism might therefore have provided the selective pressure that led to the co-evolution of phosphotransacetylase and the two-malate synthase isoenzymes. |
| Links |
PubMed Online version:10.1016/j.resmic.2005.04.008 |
| Keywords |
Acetates/metabolism; Acetyl Coenzyme A/genetics; Acetyl Coenzyme A/metabolism; Aerobiosis; Coenzyme A/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial/physiology; Gene Expression Regulation, Enzymologic/physiology; Ketoglutaric Acids/metabolism |
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