PMID:16150445
| Citation |
Encinar, JA, Molina, ML, Poveda, JA, Barrera, FN, Renart, ML, Fernández, AM and González-Ros, JM (2005) The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA. FEBS Lett. 579:5199-204 |
|---|---|
| Abstract |
The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440-446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1-125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions. |
| Links |
PubMed Online version:10.1016/j.febslet.2005.08.038 |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane/chemistry; Cell Membrane/metabolism; Detergents/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Glucosides/chemistry; Models, Molecular; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Potassium Channels/chemistry; Potassium Channels/genetics; Potassium Channels/metabolism; Protein Structure, Quaternary; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Spectroscopy, Fourier Transform Infrared; Streptomyces lividans/chemistry |
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