PMID:16120651
| Citation |
Folker, ES, Baker, BM and Goodson, HV (2005) Interactions between CLIP-170, tubulin, and microtubules: implications for the mechanism of Clip-170 plus-end tracking behavior. Mol. Biol. Cell 16:5373-84 |
|---|---|
| Abstract |
CLIP-170 belongs to a group of proteins (+TIPs) with the enigmatic ability to dynamically track growing microtubule plus-ends. CLIP-170 regulates microtubule dynamics in vivo and has been implicated in cargo-microtubule interactions in vivo and in vitro. Though plus-end tracking likely has intimate connections to +TIP function, little is known about the mechanism(s) by which this dynamic localization is achieved. Using a combination of biochemistry and live cell imaging, we provide evidence that CLIP-170 tracks microtubule plus-ends by a preassociation, copolymerization, and regulated release mechanism. As part of this analysis, we find that CLIP-170 has a stronger affinity for tubulin dimer than for polymer, and that CLIP-170 can distinguish between GTP- and GDP-like polymer. This work extends the previous analysis of CLIP-170 behavior in vivo and complements the existing fluorescence microscope characterization of CLIP-170 interactions with microtubules in vitro. In particular, these data explain observations that CLIP-170 localizes to newly polymerized microtubules in vitro but cannot track microtubule plus-ends in vitro. These observations have implications for the functions of CLIP-170 in regulating microtubule dynamics. |
| Links |
PubMed PMC1266433 Online version:10.1091/mbc.E04-12-1106 |
| Keywords |
Animals; Fluorescence Polarization; GTP-Binding Proteins/metabolism; HeLa Cells; Humans; Kymography; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Microtubules/ultrastructure; Models, Biological; Neoplasm Proteins/metabolism; Tubulin/metabolism |
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