PMID:16054140

Citation	Ahel, D, Slade, D, Mocibob, M, Söll, D and Weygand-Durasevic, I (2005) Selective inhibition of divergent seryl-tRNA synthetases by serine analogues. FEBS Lett. 579:4344-8
Abstract	Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the two types of SerRSs also differ in their recognition of the serine substrate, we compared the sensitivity of the representative methanogenic and bacterial-type SerRSs to serine hydroxamate and two previously unidentified inhibitors, serinamide and serine methyl ester. Our kinetic data showed selective inhibition of the methanogenic SerRS by serinamide, suggesting a lack of mechanistic uniformity in serine recognition between the evolutionarily distinct SerRSs.
Links	PubMed Online version:10.1016/j.febslet.2005.06.073
Keywords	Amino Acid Sequence; Archaeal Proteins/antagonists & inhibitors; Bacterial Proteins/antagonists & inhibitors; Enzyme Inhibitors/pharmacology; Escherichia coli/enzymology; Methanosarcina barkeri/enzymology; Molecular Sequence Data; Phylogeny; Serine/analogs & derivatives; Serine/pharmacology; Serine-tRNA Ligase/antagonists & inhibitors; Serine-tRNA Ligase/classification; Substrate Specificity
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