PMID:16046082
| Citation |
Hansen, T and Schönheit, P (2005) Escherichia coli phosphoglucose isomerase can be substituted by members of the PGI family, the PGI/PMI family, and the cPGI family. FEMS Microbiol. Lett. 250:49-53 |
|---|---|
| Abstract |
The Escherichia coli strain Pgi-UdhA, a mutant of the strain MG1655, is deficient in both the pgi gene and the udhA gene and cannot grow on glucose as carbon and energy source. This strain was transformed with different pET-plasmids containing archaeal or bacterial pgi, cpgi or pgi/pmi genes from the three known PGI families (PGI, PGI/PMI, cPGI). Growth could be restored upon plasmid-based expression of pgi, pgi/pmi or cpgi genes indicating that these heterologous proteins can substitute for E. coli PGI. However, complete restoration of the growth rate could not be obtained by any of the PGIs, PGI/PMIs, or cPGIs used. The data indicate that the PGI function of the three PGI families is functionally exchangeable in glycolysis. |
| Links |
PubMed Online version:10.1016/j.femsle.2005.06.042 |
| Keywords |
Archaea/enzymology; Archaea/genetics; Bacteria/enzymology; Bacteria/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Archaeal; Genes, Bacterial; Glucose-6-Phosphate Isomerase/genetics; Glucose-6-Phosphate Isomerase/metabolism; Glycolysis; Kinetics; Mannose-6-Phosphate Isomerase/genetics; Mannose-6-Phosphate Isomerase/metabolism; Mutation; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Transformation, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.