PMID:16039523

Citation	Liu, JM, Bittker, JA, Lonshteyn, M and Liu, DR (2005) Functional dissection of sRNA translational regulators by nonhomologous random recombination and in vivo selection. Chem. Biol. 12:757-67
Abstract	Small nontranslated RNAs (sRNAs) regulate a variety of biological processes. DsrA and OxyS are two E. coli sRNAs that regulate the translation of rpoS, which encodes a protein sigma factor. Due to their structural complexity, the functional dissection of sRNAs solely by designing and assaying mutants can be challenging. Here, we present a complementary approach to the study of functional RNAs, in which highly diversified RNA libraries are generated by nonhomologous random recombination (NRR) and processed efficiently by in vivo selections that link RNA activities to cell survival. When applied to DsrA and OxyS, this approach rapidly identified essential and nonessential regions of both sRNAs. Resulting hypotheses about DsrA and OxyS structure-function relationships were tested and further refined experimentally. Our findings demonstrate an efficient, unbiased approach to the functional dissection of nucleic acids.
Links	PubMed Online version:10.1016/j.chembiol.2005.05.014
Keywords	Base Sequence; Binding Sites; Escherichia coli/genetics; Escherichia coli/metabolism; Molecular Sequence Data; Protein Modification, Translational; RNA, Small Untranslated; RNA, Untranslated/chemistry; RNA, Untranslated/pharmacology; Recombination, Genetic; Sigma Factor/chemistry; Sigma Factor/genetics; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.