PMID:1603077

Citation	Holz, A, Schaefer, C, Gille, H, Jueterbock, WR and Messer, W (1992) Mutations in the DnaA binding sites of the replication origin of Escherichia coli. Mol. Gen. Genet. 233:81-8
Abstract	Mutations (base changes) were introduced into the four DnaA binding sites (DnaA boxes) of the Escherichia coli replication origin, oriC. Mutations in a single DnaA box did not impair the ability of these origins to replicate in vivo and in vitro. A combination of mutations in two DnaA boxes, R1 and R4, resulted in slower growth of the oriC plasmid-bearing host cells. DnaA protein interaction with mutant and wild-type DnaA boxes was analyzed by DNase I footprinting. Binding of DnaA protein to a mutated DnaA box R1 was not affected by a mutation in DnaA box R4 and vice versa. Mutations in DnaA boxes R1 and R4 did not modify the ability of the DnaA protein to bind to other DnaA boxes in oriC.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Chromosomes, Bacterial; Cloning, Molecular; DNA Fingerprinting; DNA Replication; DNA, Bacterial/biosynthesis; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Molecular Sequence Data; Mutation; Plasmids
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