PMID:16030245

Citation	Park, KS, Jang, YS, Lee, H and Kim, JS (2005) Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells. J. Bacteriol. 187:5496-9
Abstract	We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.
Links	PubMed PMC1196030 Online version:10.1128/JB.187.15.5496-5499.2005
Keywords	Amino Acid Sequence; Carboxy-Lyases/genetics; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Gene Expression Profiling/methods; Gene Library; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Library; Phenotype; Protein Structure, Tertiary/genetics; Temperature; Transcription Factors; Zinc Fingers/genetics
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