PMID:16014631
| Citation |
Sergiev, PV, Lesnyak, DV, Burakovsky, DE, Kiparisov, SV, Leonov, AA, Bogdanov, AA, Brimacombe, R and Dontsova, OA (2005) Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G. J. Biol. Chem. 280:31882-9 |
|---|---|
| Abstract |
Translocation catalyzed by elongation factor G occurs after the peptidyltransferase reaction on the large ribosomal subunit. Deacylated tRNA in the P-site stimulates multiple turnover GTPase activity of EF-G. We suggest that the allosteric signal from the peptidyltransferase center that activates EF-G may involve the alteration in the conformation of elongation factor binding center of the ribosome. The latter consists of the moveable GTPase-associated center and the sarcin-ricin loop that keeps its position on the ribosome during translation elongation. The position of the GTPase-associated center was altered by mutagenesis. An insertion of additional base pair at positions C1030/G1124 was lethal and affected function of EF-G, but not that of EF-Tu. Structure probing revealed a putative allosteric signal pathway connecting the P-site with the binding site of the elongation factors. The results are consistent with the different structural requirements for EF-G and EF-Tu function, where the integrity of the path between the peptidyltransferase center and both GTPase-associated center and sarcin-ricin loop is important for EF-G binding. |
| Links |
PubMed Online version:10.1074/jbc.M505670200 |
| Keywords |
Binding Sites; Conserved Sequence; Deinococcus; GTP Phosphohydrolases/chemistry; GTP Phosphohydrolases/metabolism; Haloarcula marismortui; Mutation; Nucleic Acid Conformation; Peptide Elongation Factor G/metabolism; Peptidyl Transferases/chemistry; Peptidyl Transferases/metabolism; Protein Structure, Tertiary; RNA, Ribosomal, 23S/genetics; RNA, Ribosomal, 23S/physiology; RNA, Transfer/metabolism; Ribosomes/genetics; Ribosomes/physiology |
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