PMID:15989951
| Citation |
Nowotny, M, Gaidamakov, SA, Crouch, RJ and Yang, W (2005) Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell 121:1005-16 |
|---|---|
| Abstract |
RNase H belongs to a nucleotidyl-transferase superfamily, which includes transposase, retroviral integrase, Holliday junction resolvase, and RISC nuclease Argonaute. We report the crystal structures of RNase H complexed with an RNA/DNA hybrid and a mechanism for substrate recognition and two-metal-ion-dependent catalysis. RNase H specifically recognizes the A form RNA strand and the B form DNA strand. Structure comparisons lead us to predict the catalytic residues of Argonaute and conclude that two-metal-ion catalysis is a general feature of the superfamily. In nucleases, the two metal ions are asymmetrically coordinated and have distinct roles in activating the nucleophile and stabilizing the transition state. In transposases, they are symmetrically coordinated and exchange roles to alternately activate a water and a 3'-OH for successive strand cleavage and transfer by a ping-pong mechanism. |
| Links |
PubMed Online version:10.1016/j.cell.2005.04.024 |
| Keywords |
Arabidopsis Proteins/chemistry; Arabidopsis Proteins/metabolism; Argonaute Proteins; Bacillus/chemistry; Bacillus/metabolism; Base Sequence; Catalysis; Catalytic Domain/physiology; Crystallography, X-Ray; DNA/chemistry; DNA/metabolism; Escherichia coli/chemistry; Escherichia coli/metabolism; HIV-1/chemistry; HIV-1/metabolism; Hydroxyl Radical/chemistry; Ions/chemistry; Magnesium/chemistry; Metals/chemistry; Models, Molecular; Molecular Sequence Data; Nucleic Acid Heteroduplexes/chemistry; Nucleic Acid Heteroduplexes/metabolism; Nucleic Acid Hybridization/physiology; Protein Structure, Tertiary/physiology; RNA/chemistry; RNA/metabolism; Ribonuclease H/chemistry; Ribonuclease H/metabolism; Ribonucleases/chemistry; Ribonucleases/metabolism; Substrate Specificity/physiology; Transposases/chemistry; Transposases/metabolism; Water/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.