PMID:15987891

Citation	Drew, D, Slotboom, DJ, Friso, G, Reda, T, Genevaux, P, Rapp, M, Meindl-Beinker, NM, Lambert, W, Lerch, M, Daley, DO, Van Wijk, KJ, Hirst, J, Kunji, E and De Gier, JW (2005) A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 14:2011-7
Abstract	We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for high-throughput applications. The GFP-based pipeline will facilitate the characterization of the E. coli membrane proteome and serves as an important reference for the characterization of other membrane proteomes.
Links	PubMed PMC2279312 Online version:10.1110/ps.051466205
Keywords	Escherichia coli/genetics; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/isolation & purification; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; Lactococcus lactis/genetics; Luminescent Agents/analysis; Membrane Proteins/biosynthesis; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/isolation & purification
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