PMID:15978585
| Citation |
Puan, KJ, Wang, H, Dairi, T, Kuzuyama, T and Morita, CT (2005) fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. FEBS Lett. 579:3802-6 |
|---|---|
| Abstract |
Although flavodoxin I is indispensable for Escherichia coli growth, the exact pathway(s) where flavodoxin I is essential has not been identified. We performed transposon mutagenesis of the flavodoxin I gene, fldA, in an E. coli strain that expressed mevalonate pathway enzymes and that had a point mutation in the lytB gene of the MEP pathway resulting in the accumulation of (E)-4-hydroxy-3-methylbutyl-2-enyl pyrophosphate (HMBPP). Disruption of fldA abrogated mevalonate-independent growth and dramatically decreased HMBPP levels. The fldA- mutant grew with mevalonate indicating that the essential role of flavodoxin I under aerobic conditions is in the MEP pathway. Growth was restored by fldA complementation. Since GcpE (which synthesizes HMBPP) and LytB are iron-sulfur enzymes that require a reducing system for their activity, we propose that flavodoxin is essential for GcpE and possibly LytB activity. Thus, the essential role for flavodoxin I in E. coli is in the MEP pathway for isoprenoid biosynthesis. |
| Links |
PubMed Online version:10.1016/j.febslet.2005.05.047 |
| Keywords |
Amino Acid Substitution; Aspartic Acid/genetics; Erythritol/analogs & derivatives; Erythritol/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Flavodoxin/genetics; Flavodoxin/metabolism; Genes, Essential; Genetic Complementation Test; Glycine/genetics; Mutagenesis; Sugar Phosphates/metabolism; Terpenes/metabolism |
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