PMID:1597430
| Citation |
Witkin, EM and Roegner-Maniscalco, V (1992) Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I. J. Bacteriol. 174:4166-8 |
|---|---|
| Abstract |
A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication and cell viability, as well as the PolI-dependent replication of the plasmid carrying dnaE. |
| Links | |
| Keywords |
DNA Polymerase I/genetics; DNA Polymerase I/metabolism; DNA Polymerase III/biosynthesis; DNA Polymerase III/genetics; DNA Replication/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Mutation/genetics; Plasmids/genetics; Rec A Recombinases/genetics; Temperature |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.