PMID:1597424
| Citation |
Slettan, A, Gebhardt, K, Kristiansen, E, Birkeland, NK and Lindqvist, BH (1992) Escherichia coli K-12 and B contain functional bacteriophage P2 ogr genes. J. Bacteriol. 174:4094-100 |
|---|---|
| Abstract |
The bacteriophage P2 ogr gene encodes an essential 72-amino-acid protein which acts as a positive regulator of P2 late transcription. A P2 ogr deletion phage, which depends on the supply of Ogr protein in trans for lytic growth on Escherichia coli C, has previously been constructed. E. coli B and K-12 were found to support the growth of the ogr-defective P2 phage because of the presence of functional ogr genes located in cryptic P2-like prophages in these strains. The cryptic ogr genes were cloned and sequenced. Compared with the P2 wild-type ogr gene, the ogr genes in the B and K-12 strains are conserved, containing mostly silent base substitutions. One of the base substitutions in the K-12 ogr gene results in replacement of an alanine with valine at position 57 in the Ogr protein but does not seem to affect the function of Ogr as a transcriptional activator. The cryptic ogr genes are constitutively transcribed, apparently at a higher level than the wild-type ogr gene in a P2 lysogen. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Coliphages/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial/genetics; Genes, Viral/genetics; Lysogeny/genetics; Molecular Sequence Data; Plasmids/genetics; Polymerase Chain Reaction; Transcription Factors/chemistry; Transcription Factors/genetics; Viral Proteins/chemistry; Viral Proteins/genetics |
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