PMID:15969817
| Citation |
Bresson, C, Colin, C, Chartier, F and Moulin, C (2005) Cobalt speciation study in the cobalt-cysteine system by electrospray ionization mass spectrometry and anion-exchange chromatography inductively coupled plasma atomic emission spectrometry. Appl Spectrosc 59:696-705 |
|---|---|
| Abstract |
This paper describes the ability of the combination of electrospray ionization mass spectrometry (ESI-MS) and anion-exchange chromatography coupled with inductively coupled plasma atomic emission spectrometry (AEC-ICP-AES) for cobalt speciation study in the binary cobalt-cysteine system. ESI-MS, allowing the identification and the characterization of the analytes, is used as a technique complementary to AEC-ICP-AES, providing elemental information on the separated species. The methods have been developed through the study of samples containing Co2+ and 1-fold to 5-fold molar ratios of cysteine over a pH range 2.5 to 11. In each case, cobalt-cysteine complexes were characterized by ESI-MS in negative ion mode. AEC-ICP-AES allowed further separation and detection of the cobalt species previously characterized. The strong influence of pH and ligand-to-metal ratios on the nature and stoichiometry of the species is demonstrated. For the first time, a direct experimental speciation diagram of cobalt species has been established owing to these analytical techniques. This work is a promising basis for the speciation analysis of cobalt, since a good knowledge of cobalt speciation is of prime importance to better understanding its fate in biological and environmental media. |
| Links |
PubMed Online version:10.1366/0003702053945912 |
| Keywords |
Anion Exchange Resins; Binding Sites; Chromatography, Ion Exchange/methods; Cobalt/analysis; Cobalt/chemistry; Cysteine/analysis; Cysteine/chemistry; Macromolecular Substances/analysis; Macromolecular Substances/chemistry; Spectrometry, Mass, Electrospray Ionization/methods; Spectrophotometry, Atomic/methods |
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