PMID:15933038

Citation	Raghunathan, A, Ferguson, HR Jr, Bornarth, CJ, Song, W, Driscoll, M and Lasken, RS (2005) Genomic DNA amplification from a single bacterium. Appl. Environ. Microbiol. 71:3342-7
Abstract	Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.
Links	PubMed PMC1151817 Online version:10.1128/AEM.71.6.3342-3347.2005
Keywords	Bacillus Phages/enzymology; Base Sequence; DNA, Bacterial/analysis; DNA, Bacterial/genetics; DNA-Directed DNA Polymerase/metabolism; Escherichia coli K12/cytology; Escherichia coli K12/genetics; Flow Cytometry; Genetic Techniques; Genome, Bacterial; Molecular Sequence Data; Myxococcus xanthus/cytology; Myxococcus xanthus/genetics; Nucleic Acid Amplification Techniques/methods; Polymerase Chain Reaction; RNA, Ribosomal, 16S/genetics; Sequence Analysis, DNA
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