PMID:15896790
| Citation |
Miksovská, J, Gennis, RB and Larsen, RW (2005) Photothermal studies of CO photodissociation from mixed valence Escherichia coli cytochrome bo3. FEBS Lett. 579:3014-8 |
|---|---|
| Abstract |
Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 and its transfer to CuB. The first phase occurring on a timescale of <50 ns is characterized by a volume decrease of -1.3+/-0.3 mL mol-1 and enthalpy change of 32+/-1.6 kcal mol-1. Subsequently, a volume increase of 2.9 mL mol-1 with an enthalpy change of -5.3+/-2.5 kcal mol-1 is observed with the lifetime of approximately 250 ns (this phase has not been detected in previous optical studies). These volume and enthalpy changes differ from the volume and enthalpy changes observed for CO dissociation from fully reduced cytochrome bo3 oxidase indicating that the heme o3/CuB active site dynamics are affected by the redox state of heme b. |
| Links |
PubMed Online version:10.1016/j.febslet.2005.04.055 |
| Keywords |
Carbon Monoxide/chemistry; Carbon Monoxide/metabolism; Cytochromes/chemistry; Cytochromes/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Ligands; Molecular Conformation; Oxidation-Reduction; Photolysis; Spectroscopy, Fourier Transform Infrared; Temperature; Thermodynamics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.