PMID:15879709
| Citation |
Choi, HS, Kim, KS, Park, JW, Jung, YH and Lee, Y (2005) Effects of FIS protein on rnpB transcription in Escherichia coli. Mol. Cells 19:239-45 |
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| Abstract |
Factor for inversion stimulation (FIS), the Escherichia coli protein, is a positive regulator of the transcription of genes that encode stable RNA species, such as rRNA and tRNA. Transcription of the rnpB gene encoding M1 RNA, the catalytic subunit of E. coli RNase P, rapidly declines under stringent conditions, as does that of other stable RNAs. There are multiple putative FIS binding sites upstream of the rnpB promoter. We tested whether FIS binds to these sites, and if so, how it affects rnpB transcription. In vitro binding assays revealed specific binding of FIS to multiple sites in the rnpB promoter region. Interestingly, FIS bound not only to the upstream region of the promoter, but also to the region from +4 to +18. FIS activated rnpB transcription in vitro, but the level of activation was much lower than that of the rrnB promoter for rRNA. We also examined the effects of FIS on rnpB transcription in vivo using isogenic fis+ and fis- strains. rnpB transcription was higher in the fis- than the fis+ cells during the transitions from lag to exponential phase, and from exponential to stationary phase. |
| Links | |
| Keywords |
Base Sequence; Binding Sites; DNA Footprinting; Electrophoretic Mobility Shift Assay; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Factor For Inversion Stimulation Protein/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Protein Binding; RNA, Bacterial/genetics; Transcription, Genetic/genetics; Transcriptional Activation |
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