PMID:15870475

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Citation

Plantinga, TH, van der Does, C, Tomkiewicz, D, van Keulen, G, Konings, WN and Driessen, AJ (2005) Deletion of the yiaMNO transporter genes affects the growth characteristics of Escherichia coli K-12. Microbiology (Reading, Engl.) 151:1683-9

Abstract

Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been functionally analysed. The yiaMNO genes of Escherichia coli K-12 encode one family member that transports the rare pentose l-xylulose. Its physiological role is unknown, since wild-type E. coli K-12 does not utilize l-xylulose as sole carbon source. Deletion of the yiaMNO genes in E. coli K-12 strain MC4100 resulted in remarkable changes in the transition from exponential growth to the stationary phase, high-salt survival and biofilm formation.

Links

PubMed Online version:10.1099/mic.0.27851-0

Keywords

Biofilms/growth & development; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/metabolism; Culture Media; Escherichia coli K12/drug effects; Escherichia coli K12/genetics; Escherichia coli K12/growth & development; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Operon; Signal Transduction; Sodium Chloride/pharmacology; Xylulose/metabolism

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