PMID:1586456
| Citation |
Hirose, K, Fujita, M, Takeuchi, M, Yumoto, N, Tokushige, M and Kawata, Y (1992) Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strain. Biotechnol. Appl. Biochem. 15:134-41 |
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| Abstract |
We have cloned the structural gene (tdcB) of biodegradative threonine deaminase from Escherichia coli W strain by utilizing the polymerase chain reaction. The JM109/pUCTDA strain, which was obtained by transforming E. coli JM109 with a vector plasmid (pUCTDA) containing the cloned tdcB gene, produced a large amount of the enzyme corresponding to more than 5% of the total soluble protein. Amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to the nucleotide-deduced sequence of biodegradative threonine deaminase from E. coli K-12. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Sequence Data; Polymerase Chain Reaction; Threonine Dehydratase/biosynthesis; Threonine Dehydratase/chemistry; Threonine Dehydratase/genetics |
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