PMID:15840497

Citation	Bernal, C, Mendez, E, Terencio, J, Boronat, A and Imperial, S (2005) A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity. Anal. Biochem. 340:245-51
Abstract	We report an assay for the determination of the activity of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, the enzyme which catalyzes the fourth reaction step of the 2-C-methyl-D-erythritol 4-phosphate pathway for the synthesis of isoprenoids, which is based on the spectrophotometrical determination of adenosine 5'-diphosphate using pyruvate kinase and L-lactate dehydrogenase as auxiliary enzymes. This method can be adapted to microtiter plates, can be automated, and because of its simplicity and speed can be useful for the functional characterization of the enzyme and for the screening of inhibitors with potential antibiotic or antimalarial action.
Links	PubMed Online version:10.1016/j.ab.2005.01.055
Keywords	Adenosine Diphosphate/analysis; Automation; Chromatography, High Pressure Liquid; Enzyme Inhibitors/isolation & purification; Escherichia coli/enzymology; Escherichia coli Proteins/analysis; Kinetics; L-Lactate Dehydrogenase/metabolism; Microchemistry; NAD/metabolism; Phosphoenolpyruvate/metabolism; Phosphotransferases (Alcohol Group Acceptor)/analysis; Pyruvate Kinase/metabolism; Spectrophotometry/methods
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.