PMID:15817786
| Citation |
Kershaw, CJ, Brown, NL, Constantinidou, C, Patel, MD and Hobman, JL (2005) The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations. Microbiology (Reading, Engl.) 151:1187-98 |
|---|---|
| Abstract |
The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response. |
| Links |
PubMed Online version:10.1099/mic.0.27650-0 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Copper/metabolism; Copper/pharmacology; DNA, Bacterial/genetics; Escherichia coli K12/drug effects; Escherichia coli K12/genetics; Escherichia coli K12/growth & development; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression/drug effects; Gene Expression Profiling; Genes, Bacterial/drug effects; Homeostasis/genetics; Iron/metabolism; Mutagenesis; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Polymerase Chain Reaction; Protein Kinases/genetics; Protein Kinases/metabolism; Regulon; Superoxides/metabolism; Zinc/metabolism |
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