PMID:15805537
| Citation |
Sharma, S, Davis, JA, Ayvaz, T, Traxler, B and Davidson, AL (2005) Functional reassembly of the Escherichia coli maltose transporter following purification of a MalF-MalG subassembly. J. Bacteriol. 187:2908-11 |
|---|---|
| Abstract |
Taking advantage of a chaperone-like function of MalK, a stable complex of MalF-MalG could be solubilized from the Escherichia coli membrane and purified in high yield in the absence of MalK. This MalF-MalG complex was competent for efficient reassembly of a functional MalFGK(2) maltose transporter complex both in detergent solution and in proteoliposomes. |
| Links |
PubMed PMC1070360 Online version:10.1128/JB.187.8.2908-2911.2005 |
| Keywords |
ATP-Binding Cassette Transporters/metabolism; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Maltose/metabolism; Monosaccharide Transport Proteins/metabolism |
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