PMID:15792960
| Citation |
Moritoh, S, Miki, D, Akiyama, M, Kawahara, M, Izawa, T, Maki, H and Shimamoto, K (2005) RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant Cell Physiol. 46:699-715 |
|---|---|
| Abstract |
We have cloned a new member of the RAD2/XPG nuclease family, OsGEN-L (OsGEN-like), from rice (Oryza sativa L.). OsGEN-L possesses two domains, the N- and I-regions, that are conserved in the RAD2/XPG nuclease family. Database searches and phylogenetic analyses revealed that OsGEN-L belongs to class 4 of the RAD2/XPG nuclease family, and OsGEN-L homologs were found in animals and higher plants. To elucidate the function of OsGEN-L, we generated rice OsGEN-L-RNAi transgenic plants in which OsGEN-L expression was silenced. Most of the OsGEN-L-RNAi plants displayed low fertility, and some of them were male-sterile. OsGEN-L-RNAi plants lacked mature pollen, resulting from a defect in early microspore development. A OsGEN-L-green fluorescent protein (GFP) fusion protein was localized in the nucleus, and the OsGEN-L promoter was specifically active in the anthers. Furthermore, a recombinant OsGEN-L protein possessed flap endonuclease activity and both single-stranded and double-stranded DNA-binding activities. Our results suggest that OsGEN-L plays an essential role in DNA metabolism required for early microspore development in rice. |
| Links |
PubMed Online version:10.1093/pcp/pci090 |
| Keywords |
Cell Nucleus/genetics; Cell Nucleus/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Deoxyribonucleases/genetics; Deoxyribonucleases/isolation & purification; Deoxyribonucleases/metabolism; Flap Endonucleases/genetics; Flap Endonucleases/isolation & purification; Flap Endonucleases/metabolism; Flowers/genetics; Flowers/metabolism; Gene Expression Regulation, Plant/genetics; Gene Silencing/physiology; Molecular Sequence Data; Oryza sativa/genetics; Plant Proteins/genetics; Plant Proteins/isolation & purification; Plant Proteins/metabolism; Plants, Genetically Modified; RNA Interference/physiology; RNA, Small Interfering/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Reproduction/genetics; Ribonucleases/genetics; Ribonucleases/isolation & purification; Ribonucleases/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid |
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