PMID:15782408
| Citation |
Funabashi, H, Ishikawa, M, Mie, M, Takahashi, F, Yanagida, Y, Aizawa, M and Kobatake, E (2005) Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene. Biotechnol. Bioeng. 90:509-15 |
|---|---|
| Abstract |
We developed an electrochemical detection method for evaluating cellular physiological status based on the stringent response as a means to monitor cell viability. A reporter plasmid was constructed by inserting the beta-galactosidase gene (lacZ) under the control of the rpoS promoter, and then used to transform E. coli cells. Electrochemical responses from the products catalyzed by beta-galactosidase expressed by these E. coli cells were detected using the chronoamperometric technique in a nondestructive manner. Comparisons of response currents between the relA-positive strain and relA-negative strain revealed that increases in these currents were caused by the stringent response due to the stressful alcoholic environment, and thus as a model of stressful cultivating conditions. The current was proportional to the beta-galactosidase activity assayed by a conventional method that required the destruction of cells. The cellular physiological status, which depends on the stringent response as a viability marker, therefore, could then be evaluated online with a current using the rpoS-lacZ reporter gene in the relA-positive strain without pretreatment. |
| Links |
PubMed Online version:10.1002/bit.20459 |
| Keywords |
Bacterial Proteins/genetics; Biosensing Techniques; Electrochemistry; Environmental Monitoring/methods; Escherichia coli/drug effects; Escherichia coli/genetics; Ethanol/pharmacology; Genes, Reporter; Lac Operon/genetics; Sigma Factor/genetics; beta-Galactosidase/analysis; beta-Galactosidase/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.