PMID:15774872
| Citation |
Egler, M, Grosse, C, Grass, G and Nies, DH (2005) Role of the extracytoplasmic function protein family sigma factor RpoE in metal resistance of Escherichia coli. J. Bacteriol. 187:2297-307 |
|---|---|
| Abstract |
RpoE of Escherichia coli is a sigma factor of the extracytoplasmic function protein family and is required for the expression of proteins involved in maintaining the integrity of periplasmic and outer membrane components. RpoE of E. coli was needed for full resistance to Zn(II), Cd(II), and Cu(II). Promoter gene fusion and quantitative real time reverse transcription (RT)-PCR (qRT-PCR) assays demonstrated that expression of RpoE was induced by metals. Global gene expression profiles upon metal treatment of a DeltarpoE mutant strain and its wild-type strain were analyzed with microarrays, and selected genes were confirmed by qRT-PCR. The absolute number of genes that were changed in their expression upon metal stress was similar in both strains, but the increase or decrease in transcript levels upon metal treatment was smaller in the DeltarpoE mutant strain than in the wild type. Genes showing increased expression in the DeltarpoE mutant strain encoded proteins that belong to general defense systems against protein-denaturing agents. Genes showing decreased expression were part of the RpoE modulon itself plus the ompC gene, encoding a major outer membrane protein. A DeltaompC deletion strain was as sensitive to Cu(II) and Cd(II) as the DeltarpoE mutant or a DeltarpoE DeltaompC double mutant strain. In the case of Zn(II), the double mutant was more sensitive than either single mutant. This indicates that increased expression of OmpC contributes to the RpoE modulon-mediated response to metals. |
| Links |
PubMed PMC1065229 Online version:10.1128/JB.187.7.2297-2307.2005 |
| Keywords |
Cadmium/pharmacology; Copper/pharmacology; Down-Regulation; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Gene Expression/drug effects; Metals/pharmacology; Sigma Factor/biosynthesis; Sigma Factor/genetics; Transcription Factors/biosynthesis; Transcription Factors/genetics; Up-Regulation; Zinc/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
