PMID:15769737
| Citation |
Nagaike, T, Suzuki, T, Katoh, T and Ueda, T (2005) Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. J. Biol. Chem. 280:19721-7 |
|---|---|
| Abstract |
Mammalian mitochondrial (mt) mRNAs have short poly(A) tails at their 3' termini that are post-transcriptionally synthesized by mt poly(A) polymerase (PAP). The polyadenylation of mt mRNAs is known to be a key process needed to create UAA stop codons that are not encoded in mtDNA. In some cases, polyadenylation is required for the tRNA maturation by editing of its 3' terminus. However, little is known about the functional roles the poly(A) tail of mt mRNAs plays in mt translation and RNA turnover. Here we show human mt PAP (hmtPAP) and human polynucleotide phosphorylase (hPNPase) control poly(A) synthesis in human mitochondria. Partial inactivation of hmtPAP by RNA interference using small interfering RNA in HeLa cells resulted in shortened poly(A) tails and decreased steady state levels of some mt mRNAs as well as their translational products. Moreover, knocking down hmtPAP generated markedly defective mt membrane potentials and reduced oxygen consumption. In contrast, knocking down hPNPase showed significantly extended poly(A) tails of mt mRNAs. These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs. |
| Links |
PubMed Online version:10.1074/jbc.M500804200 |
| Keywords |
Base Sequence; DNA, Complementary/genetics; HeLa Cells; Humans; Mitochondria/metabolism; Models, Molecular; Polynucleotide Adenylyltransferase/antagonists & inhibitors; Polynucleotide Adenylyltransferase/genetics; Polynucleotide Adenylyltransferase/metabolism; Polyribonucleotide Nucleotidyltransferase/metabolism; RNA/metabolism; RNA Processing, Post-Transcriptional; RNA Stability; RNA, Messenger/metabolism; RNA, Small Interfering/genetics; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Transfection |
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