PMID:15767565
| Citation |
Oh, SW, Mukhopadhyay, A, Svrzikapa, N, Jiang, F, Davis, RJ and Tissenbaum, HA (2005) JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16. Proc. Natl. Acad. Sci. U.S.A. 102:4494-9 |
|---|---|
| Abstract |
DAF-16/forkhead transcription factor, the downstream target of the insulin-like signaling in Caenorhabditis elegans, is indispensable for both lifespan regulation and stress resistance. Here, we demonstrate that c-Jun N-terminal kinase (JNK) is a positive regulator of DAF-16 in both processes. Our genetic analysis suggests that the JNK pathway acts in parallel with the insulin-like signaling pathway to regulate lifespan and both pathways converge onto DAF-16. We also show that JNK-1 directly interacts with and phosphorylates DAF-16. Moreover, in response to heat stress, JNK-1 promotes the translocation of DAF-16 into the nucleus. Our findings define an interaction between two well conserved proteins, JNK-1 and DAF-16, and provide a mechanism by which JNK regulates longevity and stress resistance. |
| Links |
PubMed PMC555525 Online version:10.1073/pnas.0500749102 |
| Keywords |
Active Transport, Cell Nucleus; Animals; Animals, Genetically Modified; Base Sequence; Caenorhabditis elegans/genetics; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; DNA, Helminth/genetics; Genes, Helminth; Longevity/genetics; Longevity/physiology; Mitogen-Activated Protein Kinase 8/genetics; Mitogen-Activated Protein Kinase 8/physiology; Models, Biological; Phosphorylation; Signal Transduction; Transcription Factors/genetics; Transcription Factors/physiology |
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