PMID:1575737
| Citation |
Ueshima, R, Fujita, N and Ishihama, A (1992) Identification of Escherichia coli proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit. Biochem. Biophys. Res. Commun. 184:634-9 |
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| Abstract |
Antisera against a synthetic tetradecameric peptide with the sequence DLIQEGNIGLMKAV, which is present in region 2.2 of both sigma 70 and sigma 32 subunits of Escherichia coli RNA polymerase, cross-reacted with more than 10 E. coli proteins including these two sigma subunits. Four major species of these cross-reacting proteins (SCRPs) were purified. N-Terminal amino acid sequence analysis revealed that one of them (SCRP-27A) was an as yet unidentified protein while the other three (SCRP-34, SCRP-27B and SCRP-23) were thioredoxin reductase, ribosomal protein S2, and alkyl hydroperoxide reductase, respectively. Immunological competition experiments with various fragments of this sigma region 2.2 peptide indicated that the anti-sigma peptide serum contained at least three different species of antibodies. All the four SCRPs analyzed here reacted with an antibody against a C-terminus-proximal epitope. |
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| Keywords |
Amino Acid Sequence; Antibodies; Bacterial Proteins/analysis; Bacterial Proteins/immunology; Blotting, Western; Cross Reactions; DNA-Directed RNA Polymerases/analysis; DNA-Directed RNA Polymerases/immunology; Escherichia coli/enzymology; Escherichia coli/metabolism; Macromolecular Substances; Molecular Sequence Data; Oligopeptides/chemical synthesis; Sequence Homology, Nucleic Acid |
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