PMID:15748982
| Citation |
Prasad Maharjan, R, Yu, PL, Seeto, S and Ferenci, T (2005) The role of isocitrate lyase and the glyoxylate cycle in Escherichia coli growing under glucose limitation. Res. Microbiol. 156:178-83 |
|---|---|
| Abstract |
Escherichia coli changes its metabolism in response to environmental circumstances, and metabolic adaptations are evident in hungry bacteria growing slowly in glucose-limited chemostats. The role of isocitrate lyase (AceA) was examined in E. coli growing under glucose limitation. AceA activity was elevated in a strain-dependent manner in the commonly used E. coli K-12 laboratory strains MG1655 and MC4100, but an aceA disruption surprisingly increased fitness under glucose limitation in both strains. However, in bacteria adapted to limiting glucose in long-term chemostats, mutations outside aceA changed its role from a negative to a positive influence. These results suggest that a recently proposed pathway of central metabolism involving the glyoxylate cycle enzymes is redundant in wild-type bacteria, but may take on a beneficial role after context adaptation. Interestingly, the aceA gene sequence did not alter during prolonged selection, so mutations in unidentified genes changed the metabolic context of unaltered AceA from a negative to a positive influence in bacteria highly adapted to limiting glucose. |
| Links |
PubMed Online version:10.1016/j.resmic.2004.09.004 |
| Keywords |
Bacterial Proteins; Biotechnology/methods; Culture Media; Directed Molecular Evolution; Escherichia coli K12/enzymology; Escherichia coli K12/genetics; Escherichia coli K12/growth & development; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Glucose/metabolism; Glyoxylates/metabolism; Heat-Shock Response; Isocitrate Lyase/genetics; Isocitrate Lyase/metabolism; Malate Synthase/metabolism; Mutation |
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