PMID:15743955
| Citation |
Wang, L, Hashimoto, Y, Tsao, CY, Valdes, JJ and Bentley, WE (2005) Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli. J. Bacteriol. 187:2066-76 |
|---|---|
| Abstract |
Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes. |
| Links |
PubMed PMC1064054 Online version:10.1128/JB.187.6.2066-2076.2005 |
| Keywords |
Bacterial Proteins/genetics; Carbon-Sulfur Lyases; Cyclic AMP/metabolism; Cyclic AMP Receptor Protein; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fermentation/physiology; Gene Expression Regulation, Bacterial; Glucose/metabolism; Homoserine/analogs & derivatives; Homoserine/biosynthesis; Homoserine/metabolism; Lactones/metabolism; Mutagenesis; N-Glycosyl Hydrolases/genetics; Operon/genetics; Promoter Regions, Genetic/physiology; Receptors, Cell Surface/genetics; Receptors, Cell Surface/metabolism; Receptors, Cyclic AMP/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
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