PMID:15715953

Citation	Datta, I, Sau, S, Sil, AK and Mandal, NC (2005) The bacteriophage lambda DNA replication protein P inhibits the oriC DNA- and ATP-binding functions of the DNA replication initiator protein DnaA of Escherichia coli. J. Biochem. Mol. Biol. 38:97-103
Abstract	Under the condition of expression of lambda P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the lambda P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the lambda P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of lambda P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.
Links	PubMed
Keywords	Adenosine Triphosphate/metabolism; Bacterial Proteins/physiology; Bacteriophage lambda/genetics; Bacteriophage lambda/metabolism; Binding Sites; DNA Replication; DNA, Bacterial/metabolism; DNA-Binding Proteins/physiology; Escherichia coli/metabolism; Escherichia coli/virology; Gene Expression Regulation, Bacterial; Genes, Lethal; Mutation/genetics; Replication Origin; Viral Proteins/physiology
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